Figure out how much bacteriostatic water to add, and exactly where to draw to on an insulin syringe for your target dose.
Peptide amount
mg / vial
Bacteriostatic water
mL added
Target dose
mcg / dose
Concentration2.5 mg/mL
Draw to10 units
on a 100-unit (1 mL) insulin syringe (example only)
What reconstitution means
Most peptides ship as a freeze-dried powder. Reconstituting means adding a liquid (almost always bacteriostatic water) back into the vial so the peptide can be measured and injected. How much water you add determines the concentration, which determines where you draw to on the syringe for a given dose.
Step by step
1Wipe the vial top and the bacteriostatic water stopper with an alcohol swab.
2Draw air into the syringe equal to the amount of water you’re adding, then inject it into the water vial to equalize pressure.
3Draw the water out, then inject it slowly down the inside wall of the peptide vial, don’t spray it directly onto the powder.
4Swirl gently to mix. Never shake, as it can damage the peptide.
5Once dissolved, label the vial with the date and refrigerate.
After mixing
Store the reconstituted vial in the refrigerator, out of direct light. Most peptides remain stable for 2–4 weeks this way (check the specific compound's page for its exact window) and should be discarded if the liquid turns cloudy or discolored.
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